A woman motorbike passenger died in a collision near Ashford in the water.
The crash between a Yamaha motorbike and a Scania lorry happened at 2.10pm on Tuesday on the A6 between Ashord and Taddington.
The rider of the bike, a man in his 40s, was taken to the The Northern General Hospital, in Sheffield, with critical injuries, where he remains in a stable condition.
The woman pillion passenger, also in her 40s, suffered fatal injuries and died in Chesterfield Royal Hospital.
Police have not yet released her name but believe she was from the West Yorkshire area.
The road was closed for over five hours while highway officers cleared debris.
A police spokesman said they were appealing for witnesses. To contact police call 0345 123 3333.