Champions Rowsley 86 were given a shock on the second matchday of their defence of the Midlands Regional Alliance, Premier Division title.
For having won their opening fixture, Rowsley were beaten 4-3 in a seven-goal thriller by Sandiacre Town, who opened their campaign in style.
After Daniel Harries and Gavin Spencer had given Sandiacre a 2-0 lead, the champions roared back to lead thanks to Nik Baker, who slotted home a penalty, and goals from Sam Hoskin and Ricky Shortt.
But Spencer pulled the home side level before completing his hat-trick to earn the Saints all three points.
Aston United remain the early-season leaders after they secured a 3-1 victory at Bestwood Miners Welfare, courtesy of a Jack Upton brace and an effort from Shay Wiggins. Jamie Crawford replied for Bestwood.
Elsewhere in the division, Stockbrook Rangers also maintained their 100 per cent start after edging past Newhall United 1-0 thanks to a first-minute strike from Aran Wightman.
In Division One, Sherwin made it two wins in two as they edged out Ripley Town 2-1 at the Racecourse Playing Fields. Goals from Ruben Francis and Paul Tyers ensured the victory for the early leaders.
Doe Lea AFC and Wirksworth Town Reserves got their first points of the season as the two sides played out a 0-0 draw at the Doe Lea Recreation Ground.
Meanwhile, Melbourne Dynamo played their first game of the new season last Wednesday night when they secured a 1-0 victory over Newhall United Reserves. Niall Worrall netted the only goal of the contest.